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CULTURIZATION
OF VIRUS
Royal
R. Rife
July
14, 1958
For
many years scientists have desired to culture
micro-organism in their filterable state. This was first
accomoplished in November of 1931 when Dr. Arthur I. Kendall,
Ph.D., Director of Medical Research, Northwestern University
Medical School succeeded in creating a media which was called
"K" media. "K" media has been demonstrated
to our entire satisfaction and has the faculties or transforming
non-filterable pathogenic micro-organisms into a state or
transition. This would be commonly represented by a hen laying
an egg whereas in this instance, the bacteria sheds a chemica1
particle which we know as a virus. Virus carry one prime
inherent factor; if the bacteria is motile, the virus is motile
and visa versa.
The
scientist will have extrema difficulty in following the
virus culturization technique. The first step in growing virus
micro-organisms is to take distilled water and make a
triple distillation as free as possible from chlorine and
flourine The Tyrode solution follows:
(1
liter of triple distilled water add-0.05 grams of Na2HPO + 8.00
grams of NaCl + 0.20 grams of KCl + 1 + 0.10 grams of CaCl2)
All
apparatus must be sterilized before use. The air factor must be
reduced as much as possible. Most virus forms become proteophilic
after being in "K" media. The filterable virus
forms have demonstrated to be the causitive agents of disease
the theoretical and applied biology cannot be denied. Standard
Berkafeld "W" and "N" filters are commonly
used which are similar to Dresden china unglazed and the
filtrate is usually drawn through with the aid of 2 inch water
vacuum. "K" medium is fundamentally made of swine
intestine. This material is relieved of all fatty substances,
boiled, and the residue and fat extracted with alcohol. This
requires at least five extractions with alcohol solution. Then
the material is dehydrated in a dehydrating oven. If this
technique is properly followed, we have from ten gallons of pig
gut from the slaughter house, one pound of dessicate.
A
combination of two grams of the dessicate and ten cubic
centimeters of Tyrode solution is placed in a test tube
accurately weighed. This procedure is duplicated as many times
as necessary depending on the contemplated tests. The test tubes
are the placed on a rack in an autoclave for three hours. The
pressure is increased very slowly to 15 psi for 1 and ½ hours
and then slowness complied with and if not, the solution will be
cloudy. Never more than two standard loops of the inoclaum is
used which is incubated at 37 ½ deg. C for 24 hours.
It
is because of the difficulty of this procedure and the lack of
specialized Rife virus microscope that observers have not been
able to see the true filterable forms of virus, bacteria, and
fungi.
The
electron microscope will not show these filterable forms as live
entities because as all scientist know, that when the
micro-organism are prepared for observation, gelatin slides, and
various stains are used which combine to show obscure forms.
These forms may be in the shape of round balls which is merely
the grannulation of the stains or in some cases part of the
micro-organism may be determined as an opaque object without
detail. We have in many instances merely taken the gelatin slide
and stained it without the filtrate and have nearly always
attained the results.
It
has been an undisputed fact over many years that these virus
could not be seen with any standard research microscope. This is
decidedly true owing to two factors; (1) the lack of
magnification, (2) improper illumination within the optical
system The Rife patented microscope lamps have
greatly improved the virus microscopes.
We
can make a positive diagnosis of any disease from which the
filtered virus form can be observed under the Rife microscopes.
The different virus mentioned in other reports have been checked
181 times against a color dictionary to see if the color of the
various forms would chance a shade or two but they have been
found to be very constant. The time for this determination
varies from five minutes to one-half hour to focus in the slide
and observe the true color and form of the virus in question,
SUMMARY
OF PROCEDURE
1.
Make media
2.
Transplant block 8 mm square of un-ulcerated malignant tissue
into 10 cc of Tyrode solution + 2 grams of "K" media
in test tube.
3.
Excite under argon filled loop at 5000 volts for 24 hours.
4.
Incubate at 37 ½ deg.. C in a 2" water bath for 24 hours.
We have found that by transplanting the initial culture through
ten transplants from "K" media to "K" media
solution (with Tyrode) with an elapsed period of 24 hours, the
virulence of the inocleum is increased. Each transplant is
incubated for 24 hours. Use one loop per transplant.
5.
Cancer virus or "BX" will grow as a cloudiness in the
solution.
6.
Examine the slide under the Rife microscopes and at 15,000x, - a
purplish red (cancer virus) virus will be seen.
7.
Inject this virus into the mammary gland of a rat no less than
40 rum under the epidermis and the cancer tumor will crow.
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