Below
are some of the Lab Notes that I received. This Laboratory was
in the process of verifying Royal Rife work. You will find these
Lab notes incomplete. As to where the rest of them are, we don't
know.
GABLES
LABORATORY
14th
Feb. 1939 6 P.M.
Received from
Dr. Scott-Wilson (Laboratories of Pathology and Public Health
Ltd.) the following stock cultures:
One of each on
slants
1. Staph. Aureus
2. " Albus 3. Pheumococcus
4. B. Influenza
5. B. Coli
6. B. Typhosum
7. Strep. Viridans
8. C. Diptheriae
9. T. B.
Received from
Dr. Parsons, the following:
One each on
slants
1. Strep. Faecalis
2. " Viridans
3. " Lactis
4. " Rheumaticus
5. Staph. Pyogenes albus
6. " Pyogenes (Insecticide Strain)
ALL THE ABOVE
CULTURES WERE PLACED IN THE INCUBATOR AT 37° C. IT IS INTENDED
TO STIMULATE GROWTH IN EACH TO MAKE READY FOR TRANSPLANTATIONS.
GABLES
LABORATORY
15th
Feb. 1939
The entire day
until 6 P.M. was taken up in the preparation of culture media.
We have on hand the following:
24 tubes Beef
Infusion Broth 12 " " " Agar 12 " Blood Agar
24 " Medium "K"
250 cc Beef
Infusion (sterile) 250 cc Tyrode Solution for Medium
"K" (sterile)
5:30 P.M.
Dr. Parsons
called and plans were made to start work on (M.O.R.) this coming
Saturday (Feb. 18th, 1939)
6:00 P.M.
Dr. Gonin came
with Dr. Brocklehurst who was shown the Rife Microscope. These
gentlemen were here for one hour.
7:00 P.M.
The following
sub-cultures were made from slants received yesterday (Dr.
Scott-Wilson) and labeled "S-W""
one each
transplanted into Beef Infusion Broth:
1. Staph. Aur.
2. " Alb. 3l Strep. Vir. 4. B. Typh. 5. B. Coli
The above were
placed in the incubator at 7:25 P.M.
GABLES
LABORATORY
16th
Feb. 1939
The following
examinations were made today of the cultures received from Dr.
Scott-Wilson; and transplants made yesterday:
B. COLI (S-W)
Slant
(original) The colonies appeared rounded, slightly raised,
rather moist grayish, and tended to coalesce.
BROTH (initial
transplant from original slant) Macroscopic: A very heavy
turbidity was noted in 15 hrs.
(a) STAINED:
All organisms were gram neg. rods and the morphology was typical
of the Colon-Typhoid group.
(b) UNSTAINED:
Each field contained numerous, moderately motile, rods. Long,
thread forms were seen occasionally.
REMARKS: A
transplant of the broth culture (apparently pure) must be made
on Mac Conkey's medium to differentiate between the Typhoid and
Colon groups.
B. Typhosum
(S-W)
Slant
(original) the colonies were minute, coalescent, and very
slightly raised.
Broth (Initial
transplant from original slant) Macroscopic: In 15 hours the
medium was clouded with a heavy growth.
Microscopic:
(a) STAINED: All organisms were gram neg.: morphologically
typical of the Typhoid-Colon group.
(b) UNSTAINED:
each filed was loaded with highly motile rod forms. The high
motility is strongly suggestive of the Typhoid- Paratyphoid
group.
REMARKS: A
transplant on Mac Conkey's medium necessary.
continued
. . . . . . . . . . .
GABLES
LABORATORY
16th
Feb. 1939
(continued
from previous page)
STREP VIRIDANS
(S-W)
No growth
apparent in broth ( 5 P.M.)
REMARKS: The
broth culture was returned to the incubator to allow organisms
to grow if they are present and conditions are favorable.
Otherwise it will be necessary to use other liquid media such as
Dextrose Brain Broth.
STAPH ALBUS
(S-W)
Slant
(original) colonies were very minute, smooth and shining,
slightly elevated, and characteristically white in color.
Broth (initial
transplant from original slant)
Macroscopic:
The media was very cloudy in 15 hours.
Microscopic:
UNSTAINED: the picture was typical of Staph.
STAINED: each
field contained numerous, gram positive cocci; occurring singly,
REMARKS: The
above reactions are sufficient to classify these organisms as
Staph Albus.
STAPH AUREUS (
S-W )
Morphology and
staining reactions same as above except that colonies on slant
were medium yellow in color instead of white.
Since we are
waiting for culture tubes and other materials, we cannot proceed
with the other organisms. The slants of these are in the
incubator at 37° C.
The following
broth cultures seem pure, and can be used for testing MOR's
Saturday:
continued
. . . . . . . . . .
GABLES
LABORATORY
16th
Feb. 1939
(continued
from prev. page)
1. Staph.
Pyogenes Albus (positive)
2. Staph. Pyogenes Aureus (positive)
3. B. Coli (not positive until MacConkey's test)
4. B. Typhosum (ditto)
5 P.M.
A transfer was
made into Beef Infusion Broth of the organisms on the slant
given us by Dr. Parsons (labeled Strep Vir). The broth
transplant was labeled "Par", and placed in the
incubator at 37° C.
GABLES
LABORATORY
Feb.
17th 1939
LABORATORY
CLOSED TODAY. CALLED ON BAIRD & TATLOCK ABOUT MATERIAL. SAW
DR. CROFTEN AT WELBECK STREET. RECEIVED CULTURE OF "FOOT
& MOUTH" BACT. FROM DR. CROFTEN AND WILL ATTEMPT TO
OBTAIN THE VIRUS OF SAME.
18th
Feb. 1939
Examined the
culture of "foot and mouth" (Dr. Croften) and noted
the following:
UNSTAINED:
motile rod forms and a few large diplococci were seen.
STAINED
(GRAM'S): All the bacilli were gram negative. The diplococci
were gram positive and averaged 2 or 3 to the field.
A subculture
was made into Beef Infusion Broth and incubated at 37° C. A
transplant was made on Beef Infusion Agar and incubated at 37°
C. Both of the above were placed in the incubator at 11: A.M.
The subculture
of Strep Vir (Par) transferred from the original slant into Beef
Infusion Broth on Feb. 16th was studied as follows:
The medium was
very turbid indicating a heavy growth.
Microscopic
examination revealed:
UNSTAINED:
Small cocci singly and in short chains. STAINED (GRAM'S): All
organism in chains were gram pos. A few of the individual cocci
were gram negative.
REMARKS; A Bile
Test must be made to differentiate between Strep Pneumococci.
The entire
afternoon was taken up in determining the MOR of Staph Albus.
This was done as follows:
A hanging drop
preparation of Staph Albus (broth subculture of S-W) was placed
under the Rife Microscope (magnification 3500 Diam). Dr.
Parsons, Mr. Siner, and Mr. Howard all agreed that the organisms
seen by each through the microscope were Staphylococci with
typical morphology. Dr. Parsons, assisted by Mr. Howard,
manipulated the controls of the
(con't
of Feb. 18th 1939)
apparatus,
while Mr. Siner studied the organisms through the microscope. As
soon as the setting "X" was reached, Siner announced
that a change had taken place and a reading was taken of the
control dial. The hanging drop was examined and no organisms
could be found. Each microscopic field was filled with amorphous
globules. All present (the above mentions three) confirmed these
findings. A different apparatus was set up by Dr. Parsons and
Mr. Howard, and the same procedure was gone through twice using
Staph Albus again. It was found that the same phenomenon
occurred each time when the setting "X" was reached.
Two subcultures
were made of Staph Albus in Beef Infusion Broth and exposed to
the ray using the same setting ("X"). These were
incubated at 37° C. (5:15 P.M.)
GABLES
LABORATORY
19th
February 1939
1:00 P.M.
The subcultures
of Staph Albus made last evening (and rayed) show no growth at
this time.
The subcultures
of "foot and mouth" showed profuse growth which
rendered the media turbid.
Gram stained
smears appeared as follows:
BROTH: Gram
negative bacilli varying in length from 3 to 8 micrns. SLANT:
The same as in broth but contained gram pos. dip. as well.
REMARKS: The
broth culture shall be thrice-plated and then transferred to
medium "X".
Transplants
were made into Beef Infusion Broth as follows: (3:15 - 3:30
P.M.)
1. Staph. Pyo
Albus (S-W) 2. Staph Pyo Aur. " 3. B. Typh " 4. B.
Coli " 5. Strep. Vir. (Parsons) 6. P. Foot & Mouth
(Croften)
Transplants
were made on Beef Infusion Agar as follows:
(3:30 - 3:45
P.M.)
continued
. . . . . . . . . . . . .
(con't
of 19th Feb. 1939)
1. B. Foot
& Mouth (Croften) 2. Strep. Vir. (Parsons)
GABLES
LABORATORY
20th
Feb. 1939
10:00 A.M.
The subcultures
of Staph. Albus made (and rayed) 18th Feb. are still negative
for growth. The medium was examined for
Hydrogen-ion-concentration and found to be PH 7.6 in both tubes.
Received a
shipment from Baird & Tatlock which included the incubator
intended for us originally. The incubator that was loaned to us,
pending the arrival of the one delivered today, was re turned to
Baird & Tatlock with the driver. We have a signed receipt on
hand to that effect. The order was short 12 tubes of Mac
Conkey's media. (marked short on invoice).
All transplants
made yesterday into Beef Infusion Broth showed good growth in
the tubes.
Gram stained
smears were made as follows" BROTH 1. Staph. Pyo Albus
(S-W) typical 2. Staph Pyo Aur " " 3. B. Typh "
" 4. B. Coli " " 5. Strep Vir (Parsons) " 6.
B. Foot & Mouth Only gram neg. bacilli were seen (no
diplococci)
AGAR SLANTS 1.
B. Foot & Mouth (Croften) same findings as in Broth 2. Strep
Vir Parsons) typical
The transplant
of Strep Vir (S-W) made into Beef Infusion Broth on 15th Feb. is
still neg for growth (4:00 ) P.M.
24 tubes of
Beef Infusion Broth containing 10% Dextrose were prepared.
A transfer was
made of the original Strep Vir. (S-W) slant into Beef Inf.
Dextrose Broth, (5:15 P.M.) & immediately incubated at 37°
C. (con't.) 4. (con't of 20th Feb. 1939)
A transplant
was made of B. Foot & Mouth (Croften) from Beef Infusion
Agar to a sterile tube of the same. This is the second plating;
one more being necessary before transferring to medium
"K".
GABLES
LABORATORY
21st
February 1939
9:30 A.M.
125 cc of Ox
Bile medium was prepared, placed in test tubes and autoclaved.
1:00 P.M.
The following
bacteriological examinations were made:
1. Strep.
Rheumaticus (par) original slant (unstained) non-motile cocci,
very small, singly, and in short chains, no chains contained
more than 6 cocci.
(stained) most
of the individual cocci were gram neg. while those in pairs or
in chains were gram pos.
A subculture
was made into Beef Infusion Broth and on Beef Agar.
2. Staph. Pyo.
Albus. (Parsons) original slant (unstained) non-motile cocci,
singly, in pairs, fours, and typical staph formation.
(stained) all
cocci were gram positive.
A subculture
was made into Beef Infusion Broth and on Beef Agar.
3. Strep. Pyo.
(Parsons) original slant (unstained) non-motile cocci singly,
pairs, and short chains.
(stained) gram
pos. cocci with morphology of Strep.
A subculture
was made into Beef-Dextrose Broth (3:30 P.M.) and incubated at
37° C.
4. Pneumococcus
(S-W) original slant (unstained) exceptionally small, non-motile
dip.
(stained) gram
pos. dip. with typical morphology, and unstained
"halo".
Subcultures
were made into Beef Broth and Beef-Dextrose Broth media.
(con't.)
5. (con't from
21st of Feb. 1939)
The culture of
"foot and mouth" was plated again for the third time
and should be ready for transfer into medium "K"
tomorrow at this time (6 P.M.)
Six tubes of
Staph. Pyo. Aur. were prepared as transplants of the broth
culture of this organism made on 15th Feb. These were incubated
(6 P.M.) at 37° C., and shall be used for tests on MOR tomorrow
at 2 P.M. using Dr. Parson's apparatus.
GABLES
LABORATORY
22nd
February 1939
10 AM
Dr. Desch
visited the laboratory and was shown the Rife Microscope. The
Doctor was here until 12:30 P.M.
1:00 P.M.
The following
bacteriological examinations were made of yesterday's
transplants:
1.
Streptococcus Rheumaticus (Par) Beef-Dextrose (from slant) Heavy
growth apparent in broth. Microscopic examination revealed the
typical strep forms. The chains were considerably longer in the
liquid media. The bile medium test indicated strep.
2.
Staphylococcus Pyogenes Albus (Par) both subcultures examined.
BEEF INFUSION
BROTH: moderate growth apparent in broth, microscopically
typical of staph.
BEEF INFUSION
AGAR: appearance on slant typical; morphology and staining the
same.
3. Strep. Pyo.
(Parsons) Beef-Dextrose Broth No growth apparent in broth.
Microscopic examination showed no organisms (centrifuge method)
The medium was checked by colorimetric method and found to be PH
6.2. From these findings it was decided that all media on hand
must be checked for PH using the colorimetric method as the
media had been adjusted by the titration method (colorimetric
apparatus not being available at the time.)
(con't
on following page)
6. (con't Feb.
22, 1939) 4. Pneumococcus (S-W) both subcultures examined BEEF
INFUSION BROTH: negative for growth. BEEF-DEXTROSE BROTH;
negative for growth The PH of the media was checked and found to
be below PH 6.4. Since Pneumococci require a PH of 7.8 it was
necessary to re-adjust the media by colorimetric method.
3:30 P.M.
Dr. Parsons and
Mr. Howard arrived and immediately commenced setting up the
apparatus. All six cultures of Staph. Pyo. Aur. (transplanted
yesterday) were examined. The media was turbid with growth, and
microscopic examination showed organisms typically Staph. The
experiment on MOR was a decided failure today since no effect on
the organisms could be seen in hanging drop. Several different
settings were tried with the same negative results. A test on
Staph Albus (which was definitely destroyed by setting
"X" on 18th Feb.) was tried with negative results.
Upon further examination it was found that the apparatus had
suffered a jolt in transit and was definitely out of adjustment.
Experimentation was stopped at 8 P.M. The above mentioned (6)
cultures of Staph Aur. were destroyed by autoclaving at 20
pounds pressure for 25 minutes.
23rd Feb. 1939
CALLED ON BAIRD
& TATLOCK TODAY IN REGARD TO FILTER APPARATUS. SELECTED
MATERIALS WITH WHICH TO CONSTRUCT AN APPROPRIATE APPARATUS . . .
RETURNED TO LABORATORY AT 3: P.M.
THE APPARATUS
WAS SET UP AND FOUND TO ANSWER THE PURPOSE ADMIRABLY.
4:00 P.M.
Re-adjusted PH
of Nutrient-Dextrose Broth to PH 7.6.
5:00 P.M.
An inventory
was taken (of all organisms on hand) which shows as follows:
IN
PURE CULTURE: Staph. Pyo. Albus (Beef Infusion Broth) Staph.
Pyo. Aur. " " " Bacterium Typhosum " "
" Strep. Pyo. (Beef-Dextrose Broth) Strep. Vir. "
" " Dip. Pneumoniae " " "
(con't)
(con't
Feb. 23, 1939)
TO BE TESTED:
Corynebacterium Diptheriae Bacterium Influenzae (Pfeiffer)
Mycobacterium Tuberculosis Hominum Streptococcus Rheumaticus
Streptococcus Lactis Streptococcus Faecalis
24th Feb. 1939
The MOR
experiments depend a great deal for results upon the
hydrogen-ion-concentration of the media. It has been
demonstrated that the slightest change in the reaction of the
media effects a corresponding change in the metabolism of the
organism. Since organisms of different metabolism react to
different vibratory rates, the necessity for absolute control of
PH in media is clearly indicated.
The entire
morning was devoted to the study of indicators and a technique
incorporating the proper indicator in media so that any change
in re-action can be noted. (during growth of organisms)
The following
tests were made:
1. 10 cc of
Beef-Dextrose Broth, of PH 7.6 (containing one drop of Phenol
Red indicator) was inoculated with three drops Bacterium
Typhosum and incubated at A subculture was made into
Beef-Dextrose Broth (3:30 P.M.) and incubated at 37° C.
2. 10 cc of
Beef Dextrose Broth, of PH 7.6, (containing one drop of
phenolpthalein indicator) was inoculated with three drops
Bacterium Typhosum and incubated at A subculture was made into
Beef-Dextrose Broth (3:30 P.M.) and incubated at 37° C.
Control tubes
were made of the above containing all except the typhoid
organisms and also incubated with the others.
It is hoped
that the amount of Phenol in the media will not affect the
growth of the organisms, and that the production of acid by the
B. Typhosum shall clear the media.
The culture of
Corynebacterium Diptheriae (S-W) on Loffler's Slant was studied
as follows:
UNSTAINED:
characteristic, non-motile, bacilli with rounded ends were seen.
The irregularity of shape and form and the numerous degenerative
forms indicate the probability that the organisms are true
diptheriae bacilli.
(con't)
(con't
24th Feb. 1939)
STAINED: The
organisms were irregular in taking the gram stain. Many
involution forms were seen, and most were gram pos.
A
subculture was made on Loffler's blood-serum media. A subculture
was made in medium "K"
6:00 P.M.
A subculture
was made into medium "K" of the thrice-plated slant of
foot and mouth (Croften), and incubated at A subculture was made
into Beef-Dextrose Broth (3:30 P.M.) and incubated at 37° C. at
6 P.M.
25th Feb. 1939
9:30 A.M.
An examination
of the B. Typb. Cultures containing the phenol indicator proved
that this method of checking PH is not satisfactory. The same
was true of the culture containing Phenolphthalein. The
organisms were definitely affected by the indicators.
After
discussing the problem with Dr. Parsons it was decided that a PH
meter would prove most satisfactory. Mr. Howard is investigating
the location of materials and shall submit a report on the
matter before the end of the week.
This afternoon
plans were made with Dr. Parsons and Mr. Howard in regard to
fitting up the electrical room. Work shall start on Tuesday, and
it is hoped that the electrical department shall be ready for
service at the beginning of next week.
5:00 P.M.
A VIRUS WAS
RECOVERED IN MEDIUM 'K' OF DR. CROFTEN'S CULTURE OF FOOT 7
MOUTH. The medial was turbid with growth and microscopic
examination (Leitz microscope) at 1,000 diam. showed the same
type of bacilli seen in other cultures. The culture in
"K" was then diluted with five times it's volume of
sterile distilled water and then passed through a berkefeld
"w" filter using 2" of (mercury) vacuum. An
examination was made of the filtrate (Leitz microscope) at 1,000
diam. and no organisms could be found, even though an extensive
search for them was made in over one hundred fields of the
hanging drop. The same slid was examined with the Rife
microscope at 8,000 diam., and when the prisms were set at
"X", each field was loaded with highly motile
elongated granules. It shall be necessary to inoculate the
filtrate into susceptible animals and the results shall
establish the status of this virus. A subculture was made of the
filtrate into "K" medium (6:30 P.M.) and incubated at
37°.
(con't
on following page)
GABLES
LABORATORY
27th
February 1939
The following
bacteriological examinations were made:
1.
Mycobacterium Tuberculosis Hominum (S-W) original slant
The appearance
of the colonies on the slant is typical of 7.3. A Ziehl-Neelsen
stained slide showed (microscopically) the typical, acid-fast,
rods.
2. Bacterium
Influenzae (S-W) original slant
The slant
seemed to be "chocolate-agar" and it was difficult to
study the character of the colonies.
MICROSCOPIC . .
. UNSTAINED: non-motile, cocco-baccili, and occasional thread
forms were seen.
STAINED: all
organisms on slide gram negative.
3.
Streptococcus Rheumaticus (S-W) see previous examinations
A bile-medium
test was carried out and lysis was not produced. This indicates
that the organisms are not Pneumococci, and may be classified as
Strep. This was the second bile test (as a check).
4.
Streptococcus Lactis (Par) original slant
This organism
was studied for morphology and staining re-action only and found
to be of strep morphology and gram positive. The pathogenicity
of this organism is questioned by Rife and his workers. Further
experimentation on Strep Lactis shall not be carried out.
5.
Streptococcus Faecalis (Par) original slant
This is another
organism of doubtful pathogenicity and is sometimes knows as the
Enterococcus. At the present no further work shall be done on
this organism.
The following
viruses (in ampules from the Rife Laboratories) were
transplanted into medium "K".
No. 48 . . . .
B. "X" No. 29 . . . . Poliomyelitis (from Strep) 37 .
. . . B. Coli 50 . . . . Hydrophobia (from "negri 38 . . .
. B. Typhosum bodies")
GABLES
LABORATORY
28th
February 1939
9:30 A.M.
500 cc of Beef
Infusion Broth was prepared with peptone and Na Cl in usual
proportions.
During the
process of adjusting the re-action of the media it was noted
that color tones in the media undergoing adjustment differed
considerably in body from the color shades of the standard
tubes. Further investigation proved that instructions for use of
the phenol-red indicator (given us by someone connected with
Baird & Tatlock) were not correct.
NOTE: The
following is copied from the Baird & Tatlock invoice on
which the Phenol-red was billed: . . . . .
Order No.
11281/222.39 22.2.39
50 cc. PHENOL
RED INDICATOR. 02%
Kindly note:
For use add 0.1
cc of indicator to 10.0 cc of the liquid under examination.
The proper
amount of indicator to be added to the liquid under examination
is 0.5 cc instead of 0.1 cc. This correct information was given
us by the Chemist connected with Baird & Tatlock.
Of course this
error on the part of Baird & Tatlock has set us back
considerably in our work, as all culture media must be
re-adjusted.
A full report
of this difficulty must be made to Baird & Tatlock
immediately.
The following
experiments were started to check the results obtained with the
foot and mouth virus.
5: P.M. : A
transplant was made on Beef Agar of the original slant rec'd
from Dr. Crofton. This shall be checked and plated (three times
in all) before transferring to "K".
The transplants
of viruses (nos. 48, 37, 38, 29, 50) into K media are negative
for growth (6: P.M.)
GABLES
LABORATORY
1st
March 1939
The entire day
was occupied in making culture media.
We have on hand
the following:
24 tubes Beef
Infusion Broth PH 7.6 350 cc " " " "
24 tubes
Nutrient Broth (Bact) PH 7.6 350 cc " " " "
"
6 P.M.
A transplant
was made of the Foot & Mouth bacillus into Beef Agar Slant.
The virus
transplants in "K" (from Rife) are still negative for
growth.
2nd March 1939
The cultures
received by us on 14th Feb. from Dr. Scott-Wilson and Dr.
Parsons have all been examined:
The
characteristics of most of the organisms are acceptable from the
standpoint of the Rife technique.
Each of the
acceptable varieties shall be transplanted into Medium
"K" (using the Rife technique) and the filtrates of
the transplants studied for viruses.
METHOD USED BY
RIFE FOR THE CULTIVATION OF FILTER PASSING VIRUS
1. The culture
under examination is studied for stability. If pleomorphic forms
are found in excess, the culture is not acceptable. The PH of
the medium must be the optimum for the organisms under
examination.
2. After it has
been demonstrated conclusively that the culture is pure, the
culture is thrice plated; the second from the first, and the
third from the second. It is important at this stage to study
each transplant. If a contamination is found or if pleomorphic
phorms are seen it is necessary to start over at Step One.
3. If the
organisms remained constant through the third plating, it can be
assumed that they are ready for transfer into K media.
"K" medium is prepared as follows:
a. Place 0.2 gm
of "K" into each test tube (1/2" x 5") b.
Fill test tubes half-way with TYRODE SOLUTION c. Sterilize in
autoclave at 15 lbs. for 15 minutes, after plugging with tight
cotton-wool stoppers.
(con't)
(con't
March 2, 1939 )
It is important
to incubate the media for 24 hours before inoculation to be sure
it is sterile.
Use three or
more loop-fulls of a 24 hour culture for inoculating
"K".
4. Transplants
in "K" should be incubated for at least 48 hours
before being removed from the incubator. If a good growth is
evident by clouding of the media at this point, remove and
prepare for filtration. If not, allow 48 hours more for growth
to appear. If growth does not appear in one week from time of
inoculation, start over from step one.
Filtration:
a. prepare
three filtering apparatus by autoclaving for at least 20 minutes
at 15 lbs. pressure. Only new Berkefeld candle filters (W)
should be used. b. dilute culture with five times it's volume of
sterile, normal saline. Transfer to an appropriate sterile test
tube and proceed with filtration using two inches of vacuum. At
no time can the pressure be increased. c. repeat process until
first filtrate has been twice filtered using sterile apparatus
each time.
5. Fill
"Wright's" capsules with the triple filtrate and store
in ice chest, leaving one at room temp. for further study.
6. Make a
hanging drop slide of the filtrate and examine under the
ordinary microscope at 1,000 diameters. No bacteria should be
visible at this stage.
7. Place three
drops of the filtrate into a tube of "K" and incubate
at 37° C. for 24 hours.
8. Make the
following examinations of the 24 hour culture:
a. study a
hanging drop slide of the filtrate under an ordinary microscope
at 1,000 diam. to make sure the filtrate is not contaminated.
b. after
consulting the Rife table for the "refractory index"
of the organism, place slide under Rife Microscope at 8,000
diam. and set wedge-shaped prisms so that the proper
monochromatic beam is coming through.
c. at this
stage the virus should appear in the field providing the
preparation has been carefully made.
(con't)
Note:
Above is all the Lab Notes that I received.
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